Life Imaging Center (LIC) Zentrum für Biosystemanalyse IMAGING-Home

Address:
Life Imaging Center
Center for Biological Systems Analysis (ZBSA)
Albert-Ludwigs-University
Habsburgerstr. 49
D-79104 Freiburg

Phone +49/761/203-2934
Fax +49/761/203-2941
E-mail: lic@imaging.uni-freiburg.de

Use for your first contact with the LIC the above mail address and fill out the LIC application sheet, which you find here.

How to reach and find us:

on the MAP: Building #7; ground floor - office rooms: 00.033 and 00.034


Important News... next MIAP Workshop (2days)

Huygens Deconvolution: Theory and Practice

20th - 21th July 2017

at the Center for Biological Systems Analysis (ZBSA) Freiburg University

Organization:   

Microscopy and Image Analysis Platform (MIAP), Freiburg
Life Imaging Center (LIC) at the Center for biological Systems Analysis (ZBSA), Freiburg

About the workshop:

Take advantage of the chance to get in contact with a powerful deconvolution software and to find the real information in a blurred image.

Aim of this course is to improve the knowledge for practical application of Huygens Deconvolution tools in image microscopy. The course is organized by experts in Microscopic Imaging of SVI (Hilversum, Netherlands) and MIAP/LIC (Freiburg). The course invites scientists, postgraduate students and postdocs as well as external guests.

The registration fee of 50€ covers finger food and refreshments.

Further information and the program will follow soon

Online registration is open! Just register here!



Open Application Development (OAD) and Automated Microscopy. Smart Image Acquisition Strategies and Automation

20th - 22th June 2017

at the Center for Biological Systems Analysis (ZBSA) Freiburg University


Organization:   

Microscopy and Image Analysis Platform (MIAP), Freiburg
Life Imaging Center (LIC) at the Center for biological Systems Analysis (ZBSA), Freiburg

About the workshop:

The ZEN Blue software is an open, flexible and powerful image acquisition platform that allows controlling a wide range of microscopes from ZEISS. Nowadays the automation of workflows and the smart linkage of image acquisition and image analysis are crucial (e.g. tracking migrating or growing cells, changing imaging parameters such as cell morphology changes). To fulfill those demands the ZEN Blue platform offers various features and options, which are combined inside a novel concept called Open Application Development (OAD).

The main components of OAD are:

  • Open CZI image data format
  • Python Scripting
  • Interfaces to ZEN
  • Experiment Feedback

The advanced workshop will give an overview about the various options allowing to automate workflows. Three use cases will be presented in talks. Different examples will be explained in detail and programmed during the hands-on sessions. The workshop is supported by two experienced programmers from Carl Zeiss Microscopy. The third day will be dedicated to test the programmed macros on 6 different microscope setups, which are driven by the ZEN Blue software.

The registration fee of 75€ covers finger food and refreshments (invoiced after the workshop)

Download further information and the preliminary program

Online registration is open now!! Click here

Registration deadline: June, 10th 2017

Due to the intense practical sessions the course will be restricted to a maximum of 20 participants

We hope to see you soon! Your MIAP Coordination Team

 


 

LIC News: General

The LIC was successful in the  DFG Call for "Core Facilities and Networks 2015" Freiburg has been awarded a grant over three years  for realization of the Microscopy and Image Analysis Platform Freiburg (MIAP-Freiburg).


MIAP-Freiburg will establish a series of interdisciplinary seminars and workshops covering in great depth special topics in microscopy and related fields. The activities, which are arranged as a cooperation of academia and industry, are targeted towards scientific users of microscopical techniques in the life and material sciences, computer scientists in the area of image analysis and software development, engineers and physicists from basic research, instrument developers in the whole area of microscopy and finally staff from imaging facilities world-wide.

 

Moreover the LIC application as an "Advanced Light Microscopy Multi Modal Single Sited Node" within EuroBioImaging was excellently reviewed by an independent International Evaluation Board and received the final ranking: Highly Recommended Expression of Interest for Euro-BioImaging Node. All the details of the review you find here.

The LIC is listed in the database Research Infrastructure Portal: RIsources of the German Research Foundation (DFG). For more details see here.

 


Overview of scientific publications from research groups done by using LIC infrastructure and support

LSM-U-2	ZEISS LSM 510 META with upright microscope Axio Imager Z1

Within the last five years (2010 – Oct 2015) the workgroups using the LIC infrastructure have published more than 256 publications (only 85% of the workgroups responded to our requests) containing images or data recorded in the LIC with a pooled number of 1943.9 Impact Factor points (mean 7.9 IF/per publication). This included 73 publications with an IF higher than 9, 51 publications IF >10 and 19 publications IF >15.

The majority of publications where in the field of Cell Biology (n=118, mean IF 8.5 ), followed by Developmental Biology (n=47, mean IF 8.1), Neurobiology (n=46, mean IF 7.3 ) and Plant Biology (n=21, mean IF 6.6). Structural Biology, connected with biological imaging is new covered in Freiburg since 2012 and therefore we reached here only n=3 but with a mean IF of 13.7


 

LIC General Information:

The Life Imaging Center (LIC) is a central core facility of the Albert-Ludwigs-University Freiburg open to members of all Faculties. Currently 250 active users from 6 Faculties (Biology; Medicine; Chemistry and Pharmacy; Mathematics and Physics; Environment and Natural Resources; and Engineering) are using the infrastructure of the LIC. The users come from app. 84 workgroups and the usage time of the microscopes in 2011 was about 23.000 hours. We have now approximately 60 - 80 new users per year and the same number leaving the facility. Outside users from Freiburg (Max Planck Institutes, Fraunhofer Gesellschaft) and from other universities are also welcome. For the details of application follow this link.

The LIC has now 14 rooms in the ZBSA with app. 400 m² (9 microscope rooms with 19 microscope set-ups, wet lab, large cell culture room, and large computer lab for 11 high-end workstations + 8 smaller desktop computers) plus 2 rooms with 3 LSMs (35 m²) in a neighboring building.

 

Take a Tour through the Life Imaging Center (LIC) (only for Windows Explorer)

 Start the tour

The Life Imaging Center, which is now one of the largest facilities of that type in Germany, was founded in 2000 by W. Driever and R. Nitschke as the central project Z2 of the SFB 592 "Signaling mechanisms in embryogenesis and organogenesis". The LIC was originally located in the Institute of Biology I and moved in April 2008 into the new building of the Zentrum für Biosystemanalyse (ZBSA) in Habsburgerstr. 49. With the move into the ZBSA, the LIC also merged with the Imaging Core Facility of the Medical Faculty founded in 2003 by R. Nitschke and M. Frotscher. The LIC works tightly together and shares equipment and software licenses with the Imaging facility of Max Planck Institute for Immunbiology and Epigenetics (MPI-IE) in Freiburg.

 


 


 


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